Acquire & Analyze

ACQUIRE & ANALYZE is a comprehensive data acquisition and analysis package designed specifically for measuring bioelectrics from small pieces of epithelial tissue or cells grown on permeable membranes across which the transepithelial voltage or current are controlled by the VCC600, VCC MC2, VCC MC6 or VCC MC8 voltage/current clamps. It...
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ACQUIRE & ANALYZE is a comprehensive data acquisition and analysis package designed specifically for measuring bioelectrics from small pieces of epithelial tissue or cells grown on permeable membranes across which the transepithelial voltage or current are controlled by the VCC600, VCC MC2, VCC MC6 or VCC MC8 voltage/current clamps. It consists of data acquisition hardware, interface cables and the ACQUIRE and ANALYZE software package. The acquire and analyze software runs in the Windows® environment and can simultaneously measure and graph current, voltage, conductance and/or resistance in 'real time' from one to eight tissues.

Acquire & Analyze is designed specifically for transepithelial electrophysiology. It is not intended to be a general purpose instrument. While it records voltage and current from up to 8 voltage/current clamp channels, it also imposes a command signal across the epithelium and calculates and plots transepithelial resistance in real time. This is a significant benefit over other software packages which require post processing with manual input to calculate TEER at each time of interest. Data acquisition is easy to initiate and generally requires only selection of a new file name, entry of the surface area based on slider used, and a reference. Data collection rate can be quickly selected from 3 user defined (typically once every 1, 10, or 20 sec) speeds by clicking an icon on the main screen. This yields very small data files relative to those of other software packages where that system must continuously sample at a high enough rate to capture any pulse imposed by the clamp instrument and independent pulse generator.

 The following table summarizes the differences for standard Ussing experiments:

 

A&A II

Competitive Products

File size

Small

Large

Calculation of TEER

Automatic

Manual

Application

Specific

General

Flexible?

Limited

Yes

Cables to Connect to VCC MC8

1

8-16

Software controls voltage/current clamp

Yes

No

Export to Excel?

Yes

Yes